【貨號(hào)】 BC-CL05【規(guī)格】 1ml/1.5ml
【保存】 2-8℃，12個(gè)月
【類(lèi)型】 陽(yáng)離子聚合物
【產(chǎn)品介紹】
BC Trans-Universal是一款高性能、高品質(zhì)的通用型基因轉(zhuǎn)染試劑，既可用于轉(zhuǎn)染質(zhì)粒DNA，又具有較強(qiáng)的RNA轉(zhuǎn)染能力。BC Trans-Universal具有不受血清影響、毒性低、穩(wěn)定性好、轉(zhuǎn)染簡(jiǎn)單易行、重復(fù)性好等優(yōu)點(diǎn)。
【應(yīng)用范圍】
BC Trans-Universal轉(zhuǎn)染試劑可適用于眾多較難轉(zhuǎn)染細(xì)胞株的DNA/siRNA轉(zhuǎn)染、瞬時(shí)轉(zhuǎn)染及穩(wěn)定轉(zhuǎn)染。適用于多種貼壁細(xì)胞，特別適用于各種較難轉(zhuǎn)細(xì)胞如L929、NIH3T3、MCF-7和A549等，均可得到較高的轉(zhuǎn)染效率，且重復(fù)性好。
【 質(zhì)粒DNA的轉(zhuǎn)染步驟】
1. 質(zhì)粒DNA的轉(zhuǎn)染:
以24孔板為例，請(qǐng)參考表1的轉(zhuǎn)染規(guī)模調(diào)整，步驟如下:
（1） 細(xì)胞接種: 每孔接種0.5~1.0×105個(gè)細(xì)胞，細(xì)胞培養(yǎng)12~24小時(shí)，使轉(zhuǎn)染時(shí)細(xì)胞密度達(dá)到60~70%融合度
（2） 質(zhì)粒稀釋: 將0.4µg質(zhì)粒稀釋于Opti-MEM培養(yǎng)基中，終體積10µL
（3） 復(fù)合物制備: 按比例取適量BC Trans-Universal稀釋于Opti-MEM培養(yǎng)基中， 終體積10µL，室溫孵育5分鐘后與質(zhì)粒稀釋液混勻
（4）室溫靜置20分鐘
（5） 每孔20µL 復(fù)合物加入細(xì)胞培養(yǎng)板中，混勻，37℃培養(yǎng)18~48小時(shí)后檢測(cè)基因表達(dá)，無(wú)需更換培養(yǎng)基
2. siRNA的轉(zhuǎn)染
轉(zhuǎn)染步驟與DNA相同，請(qǐng)參考表1的轉(zhuǎn)染規(guī)模進(jìn)行調(diào)整，所有數(shù)量和體積均是按孔計(jì)算。轉(zhuǎn)染高密度細(xì)胞可獲得高轉(zhuǎn)染效率、高表達(dá)水平和低細(xì)胞毒性。
【質(zhì)粒DNA和siRNA的轉(zhuǎn)染優(yōu)化】
可通過(guò)改變細(xì)胞密度、 DNA/siRNA濃度以及BC Trans-Universal濃度對(duì)轉(zhuǎn)染進(jìn)行優(yōu)化。保證細(xì)胞融合度在60%以上，BC Trans-Universal (µL): DNA (µg)可以在1:1和5:1之間調(diào)整；BC Trans-Universal (µL): siRNA(pmol)可以在0.02:1和0.15:1之間調(diào)整。
表1. 不同培養(yǎng)板所需轉(zhuǎn)染試劑和DNA/siRNA的用量
| 培養(yǎng)板 | | 接種 培養(yǎng)基 | Opti-MEM稀 釋后終體積 | DNA轉(zhuǎn)染 | siRNA轉(zhuǎn)染 |
| 試劑用量 | DNA | 試劑用量 | siRNA |
| 96孔板 | 0.3cm2 | 200µL | 10µL | 0.4µL | 0.2µg | 0.5µL | 7.5pmol |
| 24孔板 | 2.0cm2 | 500µL | 20µL | 0.8µL | 0.4µg | 1.0µL | 15pmol |
| 12孔板 | 4.0cm2 | 1mL | 40µL | 2µL | 1µg | 2.0µL | 30pmol |
| 6孔板 | 10.0cm2 | 2mL | 100µL | 4µL | 2µg | 4.0µL | 60pmol |
| 60mm | 20.0cm2 | 5mL | 0.2mL | 8µL | 4µg | 10µL | 100pmol |
| 10cm | 60.0cm2 | 15mL | 0.6mL | 24µL | 12µg | 30µL | 300pmol |
標(biāo)簽：細(xì)胞轉(zhuǎn)染
1、Haijun Liu,Shencun Fang,Wei Wang,etc.Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy[J].Part Fibre Toxicol.2016:1-17.
作者單位：東南大學(xué)
使用產(chǎn)品：Human MCP-1, TNF-α, TGF-β ELISA kit
2、Juan Zhang,Meng Cao,Wenwen Yang,etc.Inhibition of Glucose-6-Phosphate Dehydrogenase Could Enhance 1,4-Benzoquinone-Induced Oxidative Damage in K562 Cells[J].Oxid Med Cell Longev.2016:1-11.
作者單位：東南大學(xué)
使用產(chǎn)品：Human NADP, NADPH ELISA Kit
3、Qian-NanDi，Wei-XinCao，RunXu,etc.Chronic low-dose exposure of nonylphenol alters energy homeostasis in the reproductive system of female rats[J].Toxicology and Applied Pharmacology.2018,1(348):67-75.
作者單位：東南大學(xué)
使用產(chǎn)品：Rat E2 ELISA Kit
4、Muhammad Noman Khan,Bing Wang,Jing Wei,etc.CXCR1/2 antagonism with CXCL8/Interleukin-8 analogue CXCL8(3-72)K11R/G31P restricts lung cancer growth by inhibiting tumor cell proliferation and suppressing angiogenesis[J].Oncotarget.2015,6(25): 21315-21327.
作者單位：國(guó)立清華大學(xué)（臺(tái)灣）
使用產(chǎn)品：Human CXCL1, CXCL6, CXCL8 ELISA kit
5、Cheng Yu Wang,Xiang Dan Li,Zhi Hong Hao,etc.Insulin-like growth factor-1 improves diabetic cardiomyopathy through antioxidative and anti-inflammatory processes along with modulation of Akt/GSK-3β signaling in rats[J].Korean J Physiol Pharmacol.2016,20(6): 613-619.
作者單位：延邊大學(xué)
使用產(chǎn)品：Rat IL-1β, TNF-a ELISA kit
6、Xiaoyue Shen,Wenkui Sun,Yi Shi,etc.Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009[J].Mediators Inflamm.2015:1-12.
作者單位：南京大學(xué)
使用產(chǎn)品：Human IL-6 ELISA kit
7、Chun‐Tao Lei,Hua Su,Chen Ye,etc.The classic signalling and trans-signalling of interleukin‐6 are both injurious in podocyte under high glucose exposure[J].J Cell Mol Med.2018,22(1):251-260.
作者單位：華中科技大學(xué)
使用產(chǎn)品：Human sIL‐6R, sgp130 ELISA kit
8、Jian’an Bai,He Na,Xiumei Hua,etc.A retrospective study of NENs and miR-224 promotes apoptosis of BON-1 cells by targeting PCSK9 inhibition[J].Oncotarget.2017,8(4): 6929-6939.
作者單位：南京醫(yī)科大學(xué)附屬逸夫醫(yī)院
使用產(chǎn)品：Human glucocorticoid ELISA kit
9、Zhan Zhang, Di Wang,Shanlei Qiao,etc.Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid[J].Scientific reports.2017(7):1-10.
作者單位：南京醫(yī)科大學(xué)
使用產(chǎn)品：Rat LPS ELISA kit
10、Meng-Chen Lu,Jian-Ai Ji,Yong-Lin Jiang,etc.An inhibitor of the Keap1-Nrf2 protein-protein interaction protects NCM460 colonic cells and alleviates experimental colitis[J].Scientific reports. 2016(6):1-13.
作者單位：中國(guó)藥科大學(xué)
使用產(chǎn)品：Human IL-1β, IL-6, TNF-α, IFN-γELISA kit
11、Xiaotong Sun,Jing Wei,Yawei Tang,etc.Leptin-induced migration and angiogenesis in rheumatoid arthritis is mediated by reactive oxygen species[J].FEBS Open Bio.2017,7(12):1899-1908.
作者單位：大連醫(yī)科大學(xué)
使用產(chǎn)品：Human VEGF, IL‐1β, TNF-α ELISA kit
12、Geng Tian,Linlin Cheng,Xuewei Qi,etc.Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms[J].Int J Biol Sci. 2015,11(11):1296-1305.
作者單位：華中農(nóng)業(yè)大學(xué)
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13、Siyuan Cui,Lili Men,Yu Li,etc.Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells[J].Mediators Of Inflamm.2018:1-16.
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使用產(chǎn)品：Human ICAM-1, VCAM-1 ELISA kit
14、Peng-Tao Ren,Yuan Zhang,etc.Comparative investigation of the effects of specific antigen-sensitized DC-CIK and DC-CTL cells against B16 melanoma tumor cells[J].Mol Med Rep. 2017,15(4):1533-1538.
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使用產(chǎn)品：Human CCL19, CCL22 ELISA kit
15、Ming Ma,Lian-mei Zhao,Xing-xiao Yang,etc.p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway[J].Scientific reports.2016(6):1-13.
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使用產(chǎn)品：Human cAMP, CEA, SCC ELISA kit
16、Yucai Chen,Tianyi Yuan,Huifang Zhang,etc.Activation of Nrf2 Attenuates Pulmonary Vascular Remodeling via Inhibiting Endothelial-to-Mesenchymal Transition: an Insight from a Plant Polyphenol[J].Int J Biol Sci.2017,13(8):1067-1081.
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17、Rao Sun,Zuoxia Zhang,Yishan Lei,etc.Hippocampal activation of microglia may underlie the shared neurobiology of comorbid posttraumatic stress disorder and chronic pain[J].Mol Pain. 2016(12):1-13.
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18、Yuanyuan Zhuang,Jiabao Liu,Pei Ma,etc.Tamarixinin A Alleviates Joint Destruction of Rheumatoid Arthritis by Blockade of MAPK and NF-κB Activation[J].Front Pharmacol.2017(8):1-11.
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19、Huawen Shi,Guangchao Yang,Tongsen Zheng,etc.A preliminary study of ALPPS procedure in a rat model[J].Scientific reports. 2015(5):1-12.
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20、Bo Qiu,Ming Gong,Qi-Ting He,etc.Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1β-Induced Inflammation and Apoptosis in Chondrocytes[J].Biomed Res Int.2016:1-12.
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21、Gairu Li,Yang Xiang,Jin Zhao,etc.Saccharum Alhagi polysaccharide-1 and -2 promote the immunocompetence of RAW264.7 macrophages in vitro[J].Exp Ther Med.2018,15(4): 3556-3562.
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22、Yuanyuan Zhang,Minhui Sun,Yuwei Han,etc.The saponin DT-13 Attenuates Tumor Necrosis Factor-α-induced Vascular Inflammation Associated with Src/NF-кB/MAPK Pathway Modulation[J].Int J Biol Sci.2015,11(8):970-981.
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23、Ying Huang,Shaofeng Wang,Chunya Zhang,etc.Experimental study of the anti-atherosclerotic effect of demethylzeylasteral[J].Exp Ther Med.2017,13(6): 2787-2792.
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24、Lin Li,Ting Xiao,Fang Li,etc.Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats[J].Mol Med Rep.2017,16(2): 1715-1722.
作者單位：華南大學(xué)第一附屬醫(yī)院
使用產(chǎn)品：Rat UP ELISA kit
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27、Na Yang,Run-bin Sun,Xing-long Chen,etc.In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide,an active metabolite of berberrubine[J].Acta Pharmacol Sin.2017,38(3): 351-361.
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使用產(chǎn)品：Rat α- glucosidase ELISA kit
28、Xiaoxiao Qian,Juan Tan,Ling Liu,etc.MicroRNA-134-5p promotes high glucose-induced podocyte apoptosis by targeting bcl-2[J].Am J Transl Res.2018,10(3): 989-997.
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29、Ruiping Fan,Yuwei Han,Han Han,etc.DT-13 ameliorates TNF-α-induced nitric oxide production in the endothelium in vivo and in vitro[J].Biochemical and Biophysical Research Communications.2018,1(495):1175-1181.
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使用產(chǎn)品：Mouse nitric oxide, IL-8, MCP-1, ROS, TNFR ELISA kit
30、Yu Li,Ning Hao,Suping Zou,etc.Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway[J].Journal of Immunology Research.2018:1-9.
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使用產(chǎn)品：Mouse IL-1β, IL-6, TNF-α, NO ELISA Kit
31、Xichun Wang,Shibin Feng,Nana Ding,etc.Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis[J].Evidence-Based Complementary and Alternative Medicine,2018:1-9.
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32、JieChao,YanLv,JinChen,etc.SiO2-induced release of sVEGFRs from pulmonary macrophages[J].Respiratory Physiology & Neurobiology,2018(247):1-8.
作者單位：東南大學(xué)
使用產(chǎn)品：Human sVEGFR ELISA Kit
33、Meng-Chen Lu, Qiong Jiao,Tian Liu,etc.Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency[J].European Journal of Medicinal Chemistry.2018,1(143):1578-1589.
作者單位：中國(guó)藥科大學(xué)
使用產(chǎn)品：Mouse IL-1β, IL-18, TNF-α ELISA Kit
34、Zhengfeng Wu,Tianhong Gao,Ronglign Zhong,etc.Antihyperlipidaemic effect of triterpenic acid-enriched fraction from Cyclocarya paliurus leaves in hyperlipidaemic rats[J].Pharmaceutical Biology,2017,1(55):712-721.
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使用產(chǎn)品：Rat apoB48 ELISA Kit
35、Rao Sun,Zhibin Zhao,Jiying Feng,etc.Glucocorticoid-Potentiated Spinal Microglia Activation Contributes to Preoperative Anxiety-Induced Postoperative Hyperalgesia[J].Mol Neurobiol.2017,54(6):4316-4328.
作者單位：南京鼓樓醫(yī)院
使用產(chǎn)品： Rat CS, IL-1β, TNF-α ELISA Kit
36、Guoxiang You, Peifang Wang, Jun Hou,etc.Insights into the short-term effects of CeO2 nanoparticles on sludge dewatering and related mechanism[J].Water Research.2017,04(011):93-113.
作者單位：河海大學(xué)
使用產(chǎn)品：Plant LAP ELISA Kit
37、Peifang Wang, Guoxiang You, Jun Hou,etc.Responses of wastewater biofilms to chronic CeO2 nanoparticles exposure: Structural, physicochemical and microbial properties and potential mechanism[J].Water Research.2018:208-217.
作者單位：河海大學(xué)
使用產(chǎn)品：Plant AHL ELISA Kit
38、Yi Xu, Chao Wang, Jun Hou,etc.Strategies and relative mechanisms to attenuate the bioaccumulation and biotoxicity of ceria nanoparticles in wastewater biofilms[J].Bioresource Technology.2018:102-109.
作者單位：河海大學(xué)
使用產(chǎn)品：Plant ATP, DHA ELISA Kit
39、Junsheng Liu,Zhennan Gu,Wenwei Lu,etc.Multiple mechanisms applied by Lactobacillus pentosus AT6 to mute the lethal effects of Salmonella in a mouse model[J].Food Funct.2018, 23;9(5):2787-2795.
作者單位：江南大學(xué)
使用產(chǎn)品：Mouse CRP, LPS ELISA Kit
