一.人胰島素受體底物2(IRS-2)ELISA試劑盒詳細(xì)介紹
產(chǎn)品貨號(hào):SBJ-H1771
中文名稱(chēng):人胰島素受體底物2(IRS-2)ELISA試劑盒
英文名稱(chēng):human Insulin receptor substrate 2,IRS-2 ELISAkit
規(guī)格: 96T/48T
品牌:森貝伽
英文縮寫(xiě):IRS-2
種屬:人ELISA試劑盒
檢測(cè)波長(zhǎng):450 nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
庫(kù)存:現(xiàn)貨
保存及有效期:2-8℃,六個(gè)月
檢測(cè)目的:用于測(cè)定血清,血漿及相關(guān)液體等樣本。例如適合檢測(cè)包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。
森貝伽供應(yīng)的種屬有:人、大鼠、小鼠、豚鼠、兔子、豬犬、牛羊、雞鴨、植物ELISA試劑盒等
二.訂貨信息: 人胰島素受體底物2(IRS-2)ELISA試劑盒 貨號(hào) | 名稱(chēng) | 規(guī)格 | 檢測(cè)方法 | 價(jià)格 |
SBJ-H1771 | 人胰島素受體底物2(IRS-2)ELISA試劑盒 | 96T/48T | ELISA | 點(diǎn)擊訂購(gòu) |
森貝伽ELISA試劑盒組成有哪些?
封板膜、密封袋、酶標(biāo)包被板各一份,標(biāo)準(zhǔn)品、標(biāo)準(zhǔn)品稀釋液、酶標(biāo)試劑、樣品稀釋液、顯色劑A液、顯色劑B液、終止液、濃縮洗滌液各一瓶。
【獨(dú)特優(yōu)勢(shì)】
1、產(chǎn)品種類(lèi)齊全、質(zhì)量可靠、價(jià)格優(yōu)惠、靈敏度高、效果穩(wěn)定、易保存、操作簡(jiǎn)便
2、免費(fèi)提供產(chǎn)品最新報(bào)價(jià)、實(shí)驗(yàn)原理、產(chǎn)品用途及中英文說(shuō)明書(shū)
3、發(fā)貨及時(shí)(南京本地客戶(hù)有專(zhuān)門(mén)人員送貨上門(mén)及取標(biāo)本)
4、免費(fèi)提供ELISA代測(cè)服務(wù),森貝伽擁有自己的實(shí)驗(yàn)室(北京、南京、上海、武漢)和技術(shù)團(tuán)隊(duì),公司提供全方位的售前、售中、售后技術(shù)支持,為您解決實(shí)驗(yàn)過(guò)程中遇到的問(wèn)題。想要了解更多森貝伽實(shí)驗(yàn)代做服務(wù),請(qǐng)來(lái)電咨詢(xún)或點(diǎn)擊ELISA常見(jiàn)問(wèn)題頁(yè)面。
三.操作步驟: 四.注意事項(xiàng):1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。
2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4.請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線(xiàn),最好做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(×n×5)。
5.封板膜只限一次性使用,以避免交叉污染。
6.底物請(qǐng)避光保存。
7.嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9.本試劑不同批號(hào)組分不得混用。
10.如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn)。
五.標(biāo)準(zhǔn)曲線(xiàn)的繪制方法:
可以采用各種繪圖軟件來(lái)繪制ELISA標(biāo)準(zhǔn)曲線(xiàn),我公司以“Curve Exert1.3”軟件為例,繪制的ELISA標(biāo)準(zhǔn)曲線(xiàn)如下:
計(jì)算:以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線(xiàn),根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線(xiàn)查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)準(zhǔn)曲線(xiàn)的直線(xiàn)回歸方程式,將樣品的OD值代入方程式,計(jì)算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實(shí)際濃度
六.特別聲明:南京森貝伽生物科技有限公司長(zhǎng)期專(zhuān)業(yè)供應(yīng)各類(lèi)科研產(chǎn)品,包括ELISA試劑盒、生物試劑、標(biāo)準(zhǔn)品對(duì)照品、培養(yǎng)基、抗體、染色液染色試劑盒等優(yōu)質(zhì)產(chǎn)品。森貝伽本著客戶(hù)至上的原則為客戶(hù)提供高質(zhì)量高品質(zhì)的產(chǎn)品,公司承諾如有質(zhì)量的問(wèn)題免費(fèi)包退換(非人為因素造成的)。歡迎來(lái)電咨詢(xún)人胰島素受體底物2(IRS-2)ELISA試劑盒最新報(bào)價(jià)、實(shí)驗(yàn)原理、產(chǎn)品用途及中英文說(shuō)明書(shū)!
標(biāo)簽:人 ELISA試劑盒 人胰島素受體底物2(IRS-2)
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作者單位:東南大學(xué)
使用產(chǎn)品:Human MCP-1, TNF-α, TGF-β ELISA kit
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使用產(chǎn)品:Human IL-1β, IL-6, TNF-α, IFN-γELISA kit
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使用產(chǎn)品:Human VEGF, IL‐1β, TNF-α ELISA kit
12、Geng Tian,Linlin Cheng,Xuewei Qi,etc.Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms[J].Int J Biol Sci. 2015,11(11):1296-1305.
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14、Peng-Tao Ren,Yuan Zhang,etc.Comparative investigation of the effects of specific antigen-sensitized DC-CIK and DC-CTL cells against B16 melanoma tumor cells[J].Mol Med Rep. 2017,15(4):1533-1538.
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16、Yucai Chen,Tianyi Yuan,Huifang Zhang,etc.Activation of Nrf2 Attenuates Pulmonary Vascular Remodeling via Inhibiting Endothelial-to-Mesenchymal Transition: an Insight from a Plant Polyphenol[J].Int J Biol Sci.2017,13(8):1067-1081.
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使用產(chǎn)品:Human IL-1β, TNF-α ELISA kit
18、Yuanyuan Zhuang,Jiabao Liu,Pei Ma,etc.Tamarixinin A Alleviates Joint Destruction of Rheumatoid Arthritis by Blockade of MAPK and NF-κB Activation[J].Front Pharmacol.2017(8):1-11.
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使用產(chǎn)品:Mouse CII antibody ELISA kit
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使用產(chǎn)品:Rat NF-KB ELISA kit
20、Bo Qiu,Ming Gong,Qi-Ting He,etc.Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1β-Induced Inflammation and Apoptosis in Chondrocytes[J].Biomed Res Int.2016:1-12.
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22、Yuanyuan Zhang,Minhui Sun,Yuwei Han,etc.The saponin DT-13 Attenuates Tumor Necrosis Factor-α-induced Vascular Inflammation Associated with Src/NF-кB/MAPK Pathway Modulation[J].Int J Biol Sci.2015,11(8):970-981.
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23、Ying Huang,Shaofeng Wang,Chunya Zhang,etc.Experimental study of the anti-atherosclerotic effect of demethylzeylasteral[J].Exp Ther Med.2017,13(6): 2787-2792.
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24、Lin Li,Ting Xiao,Fang Li,etc.Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats[J].Mol Med Rep.2017,16(2): 1715-1722.
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使用產(chǎn)品:Rat UP ELISA kit
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27、Na Yang,Run-bin Sun,Xing-long Chen,etc.In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide,an active metabolite of berberrubine[J].Acta Pharmacol Sin.2017,38(3): 351-361.
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使用產(chǎn)品:Rat α- glucosidase ELISA kit
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30、Yu Li,Ning Hao,Suping Zou,etc.Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway[J].Journal of Immunology Research.2018:1-9.
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使用產(chǎn)品:Mouse IL-1β, IL-6, TNF-α, NO ELISA Kit
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使用產(chǎn)品:Mouse IL-1β, IL-18, TNF-α ELISA Kit
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使用產(chǎn)品: Rat CS, IL-1β, TNF-α ELISA Kit
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使用產(chǎn)品:Plant LAP ELISA Kit
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使用產(chǎn)品:Plant AHL ELISA Kit
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作者單位:河海大學(xué)
使用產(chǎn)品:Plant ATP, DHA ELISA Kit
39、Junsheng Liu,Zhennan Gu,Wenwei Lu,etc.Multiple mechanisms applied by Lactobacillus pentosus AT6 to mute the lethal effects of Salmonella in a mouse model[J].Food Funct.2018, 23;9(5):2787-2795.
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使用產(chǎn)品:Mouse CRP, LPS ELISA Kit